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An open-access microfluidic model for lung-specific functional studies at an air-liquid interface

Nalayanda, Divya D. and Puleo, Christopher and Fulton, William B. and Sharpe, Leilani M. and Wang, Tza-Huei and Abdullah, Fizan (2009) An open-access microfluidic model for lung-specific functional studies at an air-liquid interface. Biomedical Microdevices, 11 (5). pp. 1081-1089.

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Abstract

In an effort to improve the physiologic relevance of existing in vitro models for alveolar cells, we present a microfluidic platform which provides an air-interface in a dynamic system combining microfluidic and suspended membrane culture systems. Such a system provides the ability to manipulate multiple parameters on a single platform along with ease in cell seeding and manipulation. The current study presents a comparison of the efficacy of the hybrid system with conventional platforms using assays analyzing the maintenance of function and integrity of A549 alveolar epithelial cell monolayer cultures. The hybrid system incorporates bio-mimetic nourishment on the basal side of the epithelial cells along with an open system on the apical side of the cells exposed to air allowing for easy access for assays.

Item Type:Article
Subjects:Biomedical Science > Nanotechnology for human health
ID Code:8004
Deposited By:CSMNT
Deposited On:17 Aug 2010 14:08
Last Modified:17 Aug 2010 14:08

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