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X-ray induced damage in DNA monitored by X-ray photoelectron spectroscopy

Ptasinska, Sylwia and Stypcznyska, Agnieszka and Nixon, Tony and Mason, Nigel and Klyachko, DV and Sanche, L (2008) X-ray induced damage in DNA monitored by X-ray photoelectron spectroscopy. The Journal of Chemical Physics, 129 (6). pp. 129-134.

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Official URL: http://oro.open.ac.uk/11389/

Abstract

In this work, the chemical changes in calf thymus DNA samples were analyzed by X-ray photoelectron spectroscopy XPS. The DNA samples were irradiated for over 5 h and spectra were taken repeatedly every 30 min. In this approach the X-ray beam both damages and probes the samples. In most cases, XPS spectra have complex shapes due to contributions of C, N, and O atoms bonded at several different sites. We show that from a comparative analysis of the modification in XPS line shapes of the C 1s, O 1s, N 1s, and P 2p peaks, one can gain insight into a number ofreaction pathways leading to radiation damage to DNA.

Item Type:Article
Additional Information:Text available on ORO repository
Uncontrolled Keywords:biochemistry, biological effects of X-rays, biological techniques, DNA, molecular biophysics, spectral line breadth, X-ray photoelectron spectra
Subjects:Biomedical Science > Nanotechnology for human health
Analytical Science > Beam methods
ID Code:798
Deposited By:Lesley Tobin
Deposited On:04 Dec 2008 15:14
Last Modified:09 Feb 2009 17:05

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