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Femtosecond pulsed laser as a microscalpel for microdissection and isolation of specific sections from biological samples

Sakakura, Masaaki and Kajiyama, Shinichiro and Tsutsumi, Masafumi and Si, Jinhai and Fukusaki, Eiichiro and Tamaru, Yutaka and Akiyama, Shin-Ichi and Miura, Kiyotaka and Hirao, Kazuyuki and Ueda, Mitsuyoshi (2007) Femtosecond pulsed laser as a microscalpel for microdissection and isolation of specific sections from biological samples. JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS, 46 (9A). pp. 5859-5864.

Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.

Official URL: http://jjap.ipap.jp/link?JJAP/46/5859/

Abstract

The fermosecond laser is a candidate tool for dissecting and obtaining specific tissues or cells from biological samples. In this paper, we report on the dissection of flammable (heat-sensitive) plant tissues (Eucommia ulmoides stem) and that of a transparent zebrafish embryo placed in water. During laser dissection in the plant tissues, we observed that a low repetition rate of the irradiations is crucial for reducing the risk of thermal damage. During laser dissection of the zebrafish embryo, the laser-induced bubble formation at the tissue surface disturbed the laser-induced dissection. We concluded that the ``interior laser dissection method'', in which the dissection is performed by moving the laser spot inside a biological sample, is an efficient way to dissect a sample that is placed in water. In view of our findings, the advantages of fs laser dissection are discussed on the basis of the physical characteristics of fs laser machining.

Item Type:Article
Uncontrolled Keywords:femtosecond laser; laser dissection; tissue isolation; laser scalpel; micromanpulation; Eucommia ulmoides; zebrafish
Subjects:Biomedical Science > Nanoscale biological processes
Biomedical Science > Nanobiotechnology
Biomedical Science > Nanotechnology for animal health
Biomedical Science > Nanotechnology for human health
ID Code:796
Deposited By:Farnush Anwar
Deposited On:05 Dec 2008 12:08
Last Modified:05 Dec 2008 12:08

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