Sakakura, Masaaki and Kajiyama, Shinichiro and Tsutsumi, Masafumi and Si, Jinhai and Fukusaki, Eiichiro and Tamaru, Yutaka and Akiyama, Shin-Ichi and Miura, Kiyotaka and Hirao, Kazuyuki and Ueda, Mitsuyoshi (2007) Femtosecond pulsed laser as a microscalpel for microdissection and isolation of specific sections from biological samples. JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS, 46 (9A). pp. 5859-5864.
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Official URL: http://jjap.ipap.jp/link?JJAP/46/5859/
The fermosecond laser is a candidate tool for dissecting and obtaining specific tissues or cells from biological samples. In this paper, we report on the dissection of flammable (heat-sensitive) plant tissues (Eucommia ulmoides stem) and that of a transparent zebrafish embryo placed in water. During laser dissection in the plant tissues, we observed that a low repetition rate of the irradiations is crucial for reducing the risk of thermal damage. During laser dissection of the zebrafish embryo, the laser-induced bubble formation at the tissue surface disturbed the laser-induced dissection. We concluded that the ``interior laser dissection method'', in which the dissection is performed by moving the laser spot inside a biological sample, is an efficient way to dissect a sample that is placed in water. In view of our findings, the advantages of fs laser dissection are discussed on the basis of the physical characteristics of fs laser machining.
|Uncontrolled Keywords:||femtosecond laser; laser dissection; tissue isolation; laser scalpel; micromanpulation; Eucommia ulmoides; zebrafish|
|Subjects:||Biomedical Science > Nanoscale biological processes|
Biomedical Science > Nanobiotechnology
Biomedical Science > Nanotechnology for animal health
Biomedical Science > Nanotechnology for human health
|Deposited By:||Farnush Anwar|
|Deposited On:||05 Dec 2008 12:08|
|Last Modified:||05 Dec 2008 12:08|
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