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In Situ Loading of Basic Fibroblast Growth Factor Within Porous Silica Nanoparticles for a Prolonged Release

Zhang, Jin and Postovit, Lynne-Marie and Wang, Dashan and Gardiner, Richard B. and Harris, Richard and Abdul, Mumin Md and Thomas, Anu Alice (2009) In Situ Loading of Basic Fibroblast Growth Factor Within Porous Silica Nanoparticles for a Prolonged Release. Nanoscale Research Letters, 4 (11). pp. 1297-1302.

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Official URL: http://www.springerlink.com/content/435lq2034411u2...

Abstract

Basic fibroblast growth factor (bFGF), a protein, plays a key role in wound healing and blood vessel regeneration. However, bFGF is easily degraded in biologic systems. Mesoporous silica nanoparticles (MSNs) with well-tailored porous structure have been used for hosting guest molecules for drug delivery. Here, we report an in situ route to load bFGF in MSNs for a prolonged release. The average diameter (d) of bFGF-loaded MSNs is 57 ± 8 nm produced by a water-in-oil microemulsion method. The in vitro releasing profile of bFGF from MSNs in phosphate buffer saline has been monitored for 20 days through a colorimetric enzyme linked immunosorbent assay. The loading efficiency of bFGF in MSNs is estimated at 72.5 ± 3%. In addition, the cytotoxicity test indicates that the MSNs are not toxic, even at a concentration of 50 μg/mL. It is expected that the in situ loading method makes the MSNs a new delivery system to deliver protein drugs, e.g. growth factors, to help blood vessel regeneration and potentiate greater angiogenesis.

Item Type:Article
Uncontrolled Keywords:In situ loading method - Protein release - Nanoparticles
Subjects:Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
ID Code:7940
Deposited By:IoN
Deposited On:11 Jan 2010 15:54
Last Modified:11 Jan 2010 15:54

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