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Structure and Thickness Dependence of “Molecular Wiring” in Nanostructured Enzyme Multilayers

Flexer, Victoria and Forzani, Erica S. and Calvo, Ernesto J. and Ludueña, Silvio J. and Pietrasanta, Lia I. (2006) Structure and Thickness Dependence of “Molecular Wiring” in Nanostructured Enzyme Multilayers. Analytical Chemistry, 78 (2). p. 399. ISSN 0003-2700

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Official URL: http://dx.doi.org/10.1021/ac050973n

Abstract

Supramolecular organized multilayers composed of glucose oxidase (GOx) and osmium-derivatized poly(allylamine) redox polymer have been self-assembled electrostatically from Os−polyelectrolyte solutions of variable pH (5.5−8.8) leading to a decrease of the linear charge density in the PAH−Os with increasing pH. The layer-by-layer enzyme multilayers were studied by ellipsometry, quartz crystal microbalance, AFM, cyclic voltammetry, and electrocatalytic oxidation of β-d-glucose. At higher adsorption solution pH, an increase in the film thickness, enzyme loading, and redox charge was observed. While the electrocatalytic response increases with the increase of the adsorption solution pH (decrease of the polyelectrolyte linear charge), the FADH2 oxidation bimolecular rate constant has a maximum in the pH range 7.0−7.5 where a change in the film growth mechanism is observed.

Item Type:Article
Subjects:Material Science > Bio materials
Material Science > Nanochemistry
ID Code:7882
Deposited By:M T V
Deposited On:24 Dec 2009 11:03
Last Modified:24 Dec 2009 11:03

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