Nano Archive

Microfabricated glass devices for rapid single cell immobilization in mouse zygote microinjection

Liu, Xinyu and Sun, Yu (2009) Microfabricated glass devices for rapid single cell immobilization in mouse zygote microinjection. BIOMEDICAL MICRODEVICES, 11 (6). pp. 1169-1174. ISSN 1387-2176 (Print) 1572-8781 (Online)

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Official URL: http://www.springerlink.com/content/r7324h63370817...

Abstract

This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass bonding. Experimental results of mouse zygote immobilization and microinjection demonstrate that the device is effective for rapid cell immobilization and does not produce negative effect on embryonic development.

Item Type:Article
Subjects:Biomedical Science > Nanobiotechnology
ID Code:7829
Deposited By:M T V
Deposited On:30 Nov 2009 13:00
Last Modified:30 Nov 2009 13:00

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