Nano Archive

A self-contained fully-enclosed microfluidic cartridge for lab on a chip

Yobas, Levent and Feng Cheow, Lih and Tang, Kum-Cheong and Yong, Shien-Eit and Kye-Zheng Ong, Eleana and Wong, Lionel and Cheng-Yong Teo, William and Ji, Hongmiao and Rafeah, Siti and Yu, Chen (2009) A self-contained fully-enclosed microfluidic cartridge for lab on a chip. BIOMEDICAL MICRODEVICES, 11 (6). pp. 1279-1288. ISSN 1387-2176 (Print) 1572-8781 (Online)

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We describe a self-contained fully-enclosed cartridge for lab-on-a-chip applications where sample and reagents can be applied sequentially as is performed in a heterogeneous immunoassay, or nucleic acid extraction. Both the self-contained and fully-enclosed features of the cartridge are sought to ensure its safe use in the field by unskilled staff. Simplicity in cartridge design and operation is obtained via adopting a valveless concept whereby reagents are stored and used in the form of liquid plugs isolated by air spacers around a fluidic loop. Functional components integrated in the loop include a microfluidic chip specific to the target application, a novel peristaltic pump to displace the liquid plugs, and a pair of removable tubing segments where one is used to introduce biological sample and while the other is to collect eluant. The novel pump is fabricated through soft-lithography technique and works by pinching a planar channel under stainless-steel ball bearings that have been magnetically loaded. The utility of the cartridge is demonstrated for automated extraction and purification of nucleic acids (DNA) from a cell lysate on a battery-operated portable system. The cartridge shown here can be further extended to sample-in-answer-out diagnostic tests.

Item Type:Article
Subjects:Biomedical Science > Nanobiotechnology
ID Code:7817
Deposited By:M T V
Deposited On:30 Nov 2009 15:58
Last Modified:30 Nov 2009 15:58

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