Wu, Huei-Wen and Lin, Xi-Zhang and Hwang, Shiaw-Min and Lee, Gwo-Bin (2009) A microfluidic device for separation of amniotic fluid mesenchymal stem cells utilizing louver-array structures. BIOMEDICAL MICRODEVICES, 11 (6). pp. 1297-1307. ISSN 1387-2176 (Print) 1572-8781 (Online)
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Official URL: http://www.springerlink.com/content/155552322v886p...
Human mesenchymal stem cells can differentiate into multiple lineages for cell therapy and, therefore, have attracted considerable research interest recently. This study presents a new microfluidic device for bead and cell separation utilizing a combination of T-junction focusing and tilted louver-like structures. For the first time, a microfluidic device is used for continuous separation of amniotic stem cells from amniotic fluids. An experimental separation efficiency as high as 82.8% for amniotic fluid mesenchymal stem cells is achieved. Furthermore, a two-step separation process is performed to improve the separation efficiency to 97.1%. These results are based on characterization experiments that show that this microfluidic chip is capable of separating beads with diameters of 5, 10, 20, and 40Â Î¼m by adjusting the volume-flow-rate ratio between the flows in the main and side channels of the T-junction focusing structure. An optimal volume-flow-rate ratio of 0.5 can lead to high separation efficiencies of 87.8% and 85.7% for 5-Î¼m and 10-Î¼m beads, respectively, in a one-step separation process. The development of this microfluidic chip may be promising for future research into stem cells and for cell therapy.
|Subjects:||Biomedical Science > Nanobiotechnology|
Biomedical Science > Nanomedicine
|Deposited By:||M T V|
|Deposited On:||30 Nov 2009 16:03|
|Last Modified:||30 Nov 2009 16:03|
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