Celedon, Alfredo and Nodelman, Ilana M. and Wildt, Bridget and Dewan, Rohit and Searson, Peter and Wirtz, Denis and Bowman, Gregory D. and Sun, Sean X. (2009) Magnetic Tweezers Measurement of Single Molecule Torque. Nano letters, 9 (4). p. 1720. ISSN 1530-6984
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Official URL: http://dx.doi.org/10.1021/nl900631w
Torsional stress in linear biopolymers such as DNA and chromatin has important consequences for nanoscale biological processes. We have developed a new method to directly measure torque on single molecules. Using a cylindrical magnet, we manipulate a novel probe consisting of a nanorod with a 0.1 μm ferromagnetic segment coupled to a magnetic bead. We achieve controlled introduction of turns into the molecule and precise measurement of torque and molecule extension as a function of the number of turns at low pulling force. We show torque measurement of single DNA molecules and demonstrate for the first time measurements of single chromatin fibers.
|Subjects:||Physical Science > Nanomagnetics|
|Deposited By:||M T V|
|Deposited On:||23 Nov 2009 12:46|
|Last Modified:||23 Nov 2009 12:46|
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