Rezaei-Zarchi, S. and Saboury, A. A. and Javed, A. and Barzegar, A. and Ahmadian, S. and Bayandori-Moghaddam, A. (2008) Nano-composition of riboflavin-nafion functional film and its application in biosensing. JOURNAL OF BIOSCIENCES, 33 (2). pp. 279-287. ISSN 0250-5991
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Official URL: http://www.springerlink.com/content/pq48k5j771215w...
Abstract
A novel nafion-riboflavin membrane was constructed and characterized by the scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible spectroscopy and cyclic voltammetric techniques. The estimated average diameter of the designed nanoparticles was about 60 nm. The functional membrane showed a quasi-reversible electrochemical behaviour with a formal potential of -562 +/- 5 mV (vs Ag/AgCl) on the gold electrode. Some electrochemical parameters were estimated, indicating that the system has good and stable electron transfer properties. Moreover, horseradish peroxidase (HRP) was immobilized on the riboflavin-nafion functional membrane. The electrochemical behaviour of HRP was quasi-reversible with a formal potential of 80 +/- 5 mV (vs Ag/AgCl). The HRP in the film exhibited good catalytic activity towards the reduction of H2O2. It shows a linear dependence of its cathodic peak current on the concentration of H2O2, ranging from 10 to 300 mu M.
| Item Type: | Article |
|---|---|
| Subjects: | Physical Science > Nanophysics Physical Science > Nano objects Material Science > Nanochemistry Material Science > Nanostructured materials |
| Divisions: | Faculty of Engineering, Science and Mathematics > School of Physics Faculty of Engineering, Science and Mathematics > School of Chemistry |
| ID Code: | 7732 |
| Deposited By: | JNCASR |
| Deposited On: | 16 Nov 2009 09:40 |
| Last Modified: | 16 Nov 2009 09:40 |
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