Nano Archive

Rapid and label-free nanomechanical detection of biomarker transcripts in human RNA

Zhang, J and Lang, H. P. and Huber, F and Bietsch, A and Grange, W and Certa, U and McKendry, R and Guentgerodt, H. -J. and Hegner, M and Gerber, Christoph (2006) Rapid and label-free nanomechanical detection of biomarker transcripts in human RNA. NATURE NANOTECHNOLOGY, 1 (3). pp. 214-220.

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The availability of entire genome sequences has triggered the development of microarrays for clinical diagnostics that measure the expression levels of specific genes. Methods that involve labelling can achieve picomolar detection sensitivity, but they are costly, labour-intensive and time-consuming. Moreover, target amplification or biochemical labelling can influence the original signal. We have improved the biosensitivity of label-free cantilever-array sensors by orders of magnitude to detect mRNA biomarker candidates in total cellular RNA. Differential gene expression of the gene 1-8U, a potential marker for cancer progression or viral infections, has been observed in a complex background. The measurements provide results within minutes at the picomolar level without target amplification, and are sensitive to base mismatches. This qualifies the technology as a rapid method to validate biomarkers that reveal disease risk, disease progression or therapy response. We foreseee cantilever arrays being used as a tool to evaluate treatment response efficacy for personalized medical diagnostics.

Item Type:Article
Subjects:Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
ID Code:749
Deposited By:Farnush Anwar
Deposited On:15 Dec 2008 12:29
Last Modified:02 Mar 2009 15:42

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