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Bioconjugates of Glucose Oxidase and Gold Nanorods Based on Electrostatic Interaction with Enhanced Thermostability

Ma, Zhanfang and Ding, Teng (2009) Bioconjugates of Glucose Oxidase and Gold Nanorods Based on Electrostatic Interaction with Enhanced Thermostability. Nanoscale Research Letters, 4 (10). pp. 1236-1240. ISSN 1931-7573 (Print) 1556-276X (Online)

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Official URL: http://www.springerlink.com/content/b16q3777811778...

Abstract

Bioconjugates made up of an enzyme and gold nanorods (GNRs) were fabricated by electrostatic interactions (layer-by-layer method, LBL) between anionic glucose oxidase (GOD) and positively charged GNRs. The assembled processes were monitored by UV–Vis spectra, zeta potential measurements, and transmission electron microscopy. The enzyme activity assays of the obtained bioconjugates display a relatively enhanced thermostability behavior in contrast with that of free enzyme. Free GOD in solution only retains about 22% of its relative activity at 90 °C. Unexpectedly, the immobilized GOD on GNRs still retains about 39.3% activity after the same treatment. This work will be of significance for the biologic enhancement using other kinds of anisotropic nanostructure and suggests a new way of enhancing enzyme thermostability using anisotropic metal nanomaterials.

Item Type:Article
Uncontrolled Keywords:Gold nanorods - Enzyme thermostability - Glucose oxidase - Polyelectrolytes
Subjects:Material Science > Functional and hybrid materials
Biomedical Science > Nanobiotechnology
ID Code:7127
Deposited By:Lesley Tobin
Deposited On:13 Nov 2009 16:51
Last Modified:13 Nov 2009 16:51

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