Nano Archive

Characterizations of immobilized neutral lipase on chitosan nano-particles

Tang, Zhen-Xing and Qian, Jun-Qing and Shi, Lu-E (2007) Characterizations of immobilized neutral lipase on chitosan nano-particles. Materials Letters, 61 (1). 37 - 40.

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Official URL: http://www.sciencedirect.com/science/article/B6TX9...

Abstract

Characterizations of chitosan nano-particles prepared by the ionization gelation method were discussed in this paper. The optimal pH of an immobilized enzyme was 7.5. The optimal temperature of the immobilized enzyme was 45 °C. The thermal, operational and storage stabilities of an enzyme were improved after they were immobilized on chitosan nano-particles. The chitosan nano-particles were applied to protect the activity of free neutral lipase. They could improve the enzyme activity by 13.17% than that of the free neutral lipase. The Michaelis constant Km of the immobilized enzyme was disclosed as 0.37 × 102 g/l by the Line-weaver Burk plot at 40 °C.

Item Type:Article
Uncontrolled Keywords:Chitosan; Nano-particles; Characterizations; Enzyme; Immobilization
Subjects:Biomedical Science > Nanobiotechnology
Material Science > Nanostructured materials
ID Code:6761
Deposited By:SPI
Deposited On:09 Feb 2010 09:10
Last Modified:09 Feb 2010 09:10

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