Tang, Zhen-Xing and Qian, Jun-Qing and Shi, Lu-E (2007) Characterizations of immobilized neutral lipase on chitosan nano-particles. Materials Letters, 61 (1). 37 - 40.
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Characterizations of chitosan nano-particles prepared by the ionization gelation method were discussed in this paper. The optimal pH of an immobilized enzyme was 7.5. The optimal temperature of the immobilized enzyme was 45 °C. The thermal, operational and storage stabilities of an enzyme were improved after they were immobilized on chitosan nano-particles. The chitosan nano-particles were applied to protect the activity of free neutral lipase. They could improve the enzyme activity by 13.17% than that of the free neutral lipase. The Michaelis constant Km of the immobilized enzyme was disclosed as 0.37 × 102 g/l by the Line-weaver Burk plot at 40 °C.
|Uncontrolled Keywords:||Chitosan; Nano-particles; Characterizations; Enzyme; Immobilization|
|Subjects:||Biomedical Science > Nanobiotechnology|
Material Science > Nanostructured materials
|Deposited On:||09 Feb 2010 09:10|
|Last Modified:||09 Feb 2010 09:10|
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