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On the Enhanced Antibacterial Activity of Antibiotics Mixed with Gold Nanoparticles

Burygin, G. L. and Khlebtsov, B. N. and Shantrokha, A. N. and Dykman, L. A. and Bogatyrev, V. A. and Khlebtsov, N. G. (2009) On the Enhanced Antibacterial Activity of Antibiotics Mixed with Gold Nanoparticles. Nanoscale Research Letters, 4 (8). pp. 794-801.


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The bacterial action of gentamicin and that of a mixture of gentamicin and 15-nm colloidal-gold particles on Escherichia coli K12 was examined by the agar-well-diffusion method, enumeration of colony-forming units, and turbidimetry. Addition of gentamicin to colloidal gold changed the gold color and extinction spectrum. Within the experimental errors, there were no significant differences in antibacterial activity between pure gentamicin and its mixture with gold nanoparticles (NPs). Atomic absorption spectroscopy showed that upon application of the gentamicin-particle mixture, there were no gold NPs in the zone of bacterial-growth suppression in agar. Yet, free NPs diffused into the agar. These facts are in conflict with the earlier findings indicating an enhancement of the bacterial activity of similar gentamicin–gold nanoparticle mixtures. The possible causes for these discrepancies are discussed, and the suggestion is made that a necessary condition for enhancement of antibacterial activity is the preparation of stable conjugates of NPs coated with the antibiotic molecules.

Item Type:Article
Uncontrolled Keywords:Colloidal-gold nanoparticles - Gentamicin - Drug delivery - Antibacterial activity - Agar-well-diffusion method - Minimum inhibitory concentration - Maximum tolerant concentration - Atomic absorption spectroscopy
Subjects:Biomedical Science > Nanomedicine
ID Code:6535
Deposited By:SPI
Deposited On:27 Jul 2009 16:31
Last Modified:29 Jul 2009 10:19

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