Nano Archive

Membrane Surface Nanostructures and Adhesion Property of T Lymphocytes Exploited by AFM

Wu, Yangzhe and Lu, Hongsong and Cai, Jiye and He, Xianhui and Hu, Yi and Zhao, HongXia and Wang, Xiaoping (2009) Membrane Surface Nanostructures and Adhesion Property of T Lymphocytes Exploited by AFM. Nanoscale Research Letters, 4 (8). pp. 942-947.

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Official URL: http://www.springerlink.com/content/2j261311m8ujl0...

Abstract

The activation of T lymphocytes plays a very important role in T-cell-mediated immune response. Though there are many related literatures, the changes of membrane surface nanostructures and adhesion property of T lymphocytes at different activation stages have not been reported yet. However, these investigations will help us further understand the biophysical and immunologic function of T lymphocytes in the context of activation. In the present study, the membrane architectures of peripheral blood T lymphocytes were obtained by AFM, and adhesion force of the cell membrane were measured by acquiring force–distance curves. The results indicated that the cell volume increased with the increases of activation time, whereas membrane surface adhesion force decreased, even though the local stiffness for resting and activated cells is similar. The results provided complementary and important data to further understand the variation of biophysical properties of T lymphocytes in the context of in vitro activation.

Item Type:Article
Uncontrolled Keywords:T lymphocytes - Cell activation - Membrane nanostructures - Adhesion force
Subjects:Biomedical Science > Nanobiotechnology
ID Code:6511
Deposited By:SPI
Deposited On:28 Jul 2009 10:52
Last Modified:28 Jul 2009 10:52

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