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The interaction of the protein lysozyme with bacteria E-coli observed using nanodiamond labelling

Perevedentseva, Elena and Cheng, Chih-Yuan and Chung, Pei-Hua and Tu, Jhih-Sian and Hsieh, Yu-Hsin and Cheng, Chia-Liang (2007) The interaction of the protein lysozyme with bacteria E-coli observed using nanodiamond labelling. NANOTECHNOLOGY, 18 (31).

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Official URL: http://www.iop.org/EJ/abstract/0957-4484/18/31/315...

Abstract

The application of a nanometre-sized diamond in Raman-detectable biolabelling is demonstrated in this study. The interaction of a lysozyme-nanodiamond complex with bacteria E. coli was observed via Raman mapping using the diamond Raman signal as the labelling marker. The results are compared with scanning electron microscope observations, and the adsorbed lysozyme's functionality is analysed. High antibacterial activity of lysozyme-nanodiamond complex was observed, equivalent to active lysozyme in solution. The results suggest that nanodiamond labelling can be effective and that it can be applied in ambient conditions without complicated sample pre-treatments.

Item Type:Article
Uncontrolled Keywords:ENHANCED RAMAN-SCATTERING; DIAMOND SURFACES; SPECTROSCOPY; IMMOBILIZATION; ABSORPTION; ADSORPTION; DESORPTION; OXIDATION; BINDING
Subjects:Biomedical Science > Nanobiotechnology
ID Code:6457
Deposited By:IoN
Deposited On:23 Sep 2009 11:24
Last Modified:23 Sep 2009 11:24

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