Escalante, Maryana and Maury, Pascale and Bruinink, Christiaan M. and van der Werf, Kees and Olsen, John D. and Timney, John A. and Huskens, Jurriaan and Hunter, C. Neil and Subramaniam, Vinod and Otto, Cees (2008) Directed assembly of functional light harvesting antenna complexes onto chemically patterned surfaces. NANOTECHNOLOGY, 19 (2).
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Official URL: http://www.iop.org/EJ/abstract/-search=66091837.1/...
We report the directed assembly of the photosynthetic membrane proteins LH1 and LH2 isolated from the purple bacterium Rhodobacter sphaeroides onto chemically patterned substrates. Nanoimprint lithography was used to pattern discrete regions of amino- and fluoro-terminated or poly( ethylene glycol) self-assembled monolayers onto a glass substrate. Densely packed layers of assembled protein complexes were observed with atomic force microscopy. The protein complexes attached selectively to the amino- terminated regions by electrostatic interactions. Spectral images generated with a hybrid scanning probe and fluorescence microscope confirmed that the patterned proteins retained their native optical signatures.
|Uncontrolled Keywords:||CAPILLARY FORCE LITHOGRAPHY; NANOIMPRINT LITHOGRAPHY; PROTEIN IMMOBILIZATION; IMPRINT LITHOGRAPHY; CRYSTAL-STRUCTURE; FABRICATION; MICROSCOPY; MONOLAYERS; NANOLITHOGRAPHY; PHOTOSYNTHESIS|
|Subjects:||Analytical Science > Microscopy and probe methods|
Biomedical Science > Nanobiotechnology
|Deposited On:||24 Sep 2009 12:47|
|Last Modified:||24 Sep 2009 12:47|
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