Nano Archive

PC12 differentiation on biopolymer nanostructures

Cecchini, Marco and Bumma, Giorgia and Serresi, Michela and Beltram, Fabio (2007) PC12 differentiation on biopolymer nanostructures. NANOTECHNOLOGY, 18 (50).

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The study of nervous system regeneration and axonal outgrowth control are relevant in several research areas, like neurophysiology or biomedical engineering. Among the elements that control neuron dynamics, the host substrate topography is a key parameter in determining cell differentiation. We present time-lapse experiments to analyze the differentiation dynamics of PC 12 cells on nanopatterned biocompatible substrates. 200 nm depth gratings were fabricated on tissue-culture polystyrene substrates by nanoimprint lithography; different linewidths and pitches were compared down to 500 nm and 1000 nm, respectively. PC12 cells were cultured on these substrates and, following NGF administration to the medium, body morphology, cell movement and neuritogenesis were monitored at different time periods. In addition to demonstrating guided differentiation, our studies show complex time variations in body morphology and axon length, and guided cell movement. We show unstable synaptic connections and cell-body polarization, and the competition between topographical guidance and cell-cell interactions.

Item Type:Article
Subjects:Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
ID Code:6324
Deposited By:IoN
Deposited On:09 Dec 2009 11:15
Last Modified:09 Dec 2009 11:15

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