Schoch, Reto B. and Cheow, Lih Feng and Han, Jongyoon (2007) Electrical detection of fast reaction kinetics in nanochannels with an induced flow. NANO LETTERS, 7 (12). pp. 3895-3900.
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Official URL: http://pubs.acs.org/doi/abs/10.1021/nl0724788
Nanofluidic channels can be used to enhance surface binding reactions, since the target molecules are closely confined to the surfaces that are coated with specific binding partners. Moreover, diffusion-limited binding can be significantly enhanced if the molecules are steered into the nanochannels via either pressure-driven or electrokinetic flow. By monitoring the nanochannel impedance, which is sensitive to surface binding, low analyte concentrations have been detected electrically in nanofluidic channels within response times of 1−2 h. This represents a 54 fold reduction in the response time using convective flow compared to diffusion-limited binding. At high-flow velocities, the presented method of reaction kinetics enhancement is potentially limited by force-induced dissociations of the receptor−ligand bonds. Optimization of this scheme could be useful for label-free, electrical detection of biomolecule binding reactions within nanochannels on a chip.
|Subjects:||Analytical Science > Nanotechnology for sensing and actuating|
|Deposited On:||07 Aug 2009 11:50|
|Last Modified:||07 Aug 2009 11:50|
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