Fore, Samantha and Yuen, Yin and Hesselink, Lambertus and Huser, Thomas (2007) Pulsed-interleaved excitation FRET measurements on single duplex DNA molecules inside C-shaped nanoapertures. NANO LETTERS, 7 (6). pp. 1749-1756.
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Official URL: http://pubs.acs.org/doi/abs/10.1021/nl070822v
Single-molecule fluorescence resonant energy transfer (FRET) is a widely accepted method for determining the spatial separation between molecules. In combination with pulsed interleaved excitation (PIE), additional information about the stoichiometry of molecular interactions is obtained. PIE-FRET, however, as implemented with standard confocal optics, requires the dilution of the sample to biologically low concentrations. Here, we show that PIE-FRET measurements inside nanometer-sized apertures yield meaningful biochemical data at 1000x higher concentrations.
|Uncontrolled Keywords:||fluorescence resonant energy transfer - pulsed interleaved excitation - confocal optics|
|Subjects:||Biomedical Science > Nanobiotechnology|
|Deposited On:||07 Sep 2009 13:49|
|Last Modified:||07 Sep 2009 13:49|
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