Nano Archive

Pulsed-interleaved excitation FRET measurements on single duplex DNA molecules inside C-shaped nanoapertures

Fore, Samantha and Yuen, Yin and Hesselink, Lambertus and Huser, Thomas (2007) Pulsed-interleaved excitation FRET measurements on single duplex DNA molecules inside C-shaped nanoapertures. NANO LETTERS, 7 (6). pp. 1749-1756.

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Official URL: http://pubs.acs.org/doi/abs/10.1021/nl070822v

Abstract

Single-molecule fluorescence resonant energy transfer (FRET) is a widely accepted method for determining the spatial separation between molecules. In combination with pulsed interleaved excitation (PIE), additional information about the stoichiometry of molecular interactions is obtained. PIE-FRET, however, as implemented with standard confocal optics, requires the dilution of the sample to biologically low concentrations. Here, we show that PIE-FRET measurements inside nanometer-sized apertures yield meaningful biochemical data at 1000x higher concentrations.

Item Type:Article
Uncontrolled Keywords:fluorescence resonant energy transfer - pulsed interleaved excitation - confocal optics
Subjects:Biomedical Science > Nanobiotechnology
ID Code:6226
Deposited By:IoN
Deposited On:07 Sep 2009 13:49
Last Modified:07 Sep 2009 13:49

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