Lei, Chenghong and Shin, Yongsoon and Liu, Jun and Ackerman, Eric J. (2007) Synergetic effects of nanoporous support and urea on enzyme activity. NANO LETTERS, 7 (4). pp. 1050-1053.
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Official URL: http://pubs.acs.org/doi/abs/10.1021/nl070255b
We report synergetic effects of functionalized mesoporous silica (FMS) and urea to promote favorable protein conformational changes. The specific activity of glucose isomerase (GI) entrapped in FMS in the presence of urea was approximately double that of GI in solution in the absence of urea. Rather than losing all activity in a denaturing solution of 8.0 M urea, the specific activity of GI entrapped in FMS remained higher than the highest specific activity of GI free in solution.
|Uncontrolled Keywords:||MESOPOROUS MOLECULAR-SIEVES; SOL-GEL PROCESS; CONFORMATIONAL FLEXIBILITY; IMMOBILIZED ENZYMES; CATALYTIC ACTIVITY; GLUCOSE-ISOMERASE; SILICA; ENCAPSULATION; ACTIVATION; PROTEINS|
|Subjects:||Biomedical Science > Nanobiotechnology|
|Deposited On:||08 Sep 2009 11:14|
|Last Modified:||08 Sep 2009 11:14|
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