Nabiev, Igor and Mitchell, Siobhan and Davies, Anthony and Williams, Yvonne and Kelleher, Dermot and Moore, Richard and Gun'ko, Yurii K. and Byrne, Stephen and Rakovich, Yury P. and Donegan, John F. and Sukhanova, Alyona and Conroy, Jennifer and Cottell, David and Gaponik, Nikolai and Rogach, Andrey and Volkov, Yuri (2007) Nonfunctionalized nanocrystals can exploit a cell's active transport machinery delivering them to specific nuclear and cytoplasmic compartments. NANO LETTERS, 7 (11). pp. 3452-3461.
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Official URL: http://pubs.acs.org/doi/abs/10.1021/nl0719832
We use high content cell analysis, live cell fluorescent imaging, and transmission electron microscopy approaches combined with inhibitors of cellular transport and nuclear import to conduct a systematic study of the mechanism of interaction of nonfunctionalized quantum dots (QDs) with live human blood monocyte-derived primary macrophages and cell lines of phagocytic, epithelial, and endothelial nature. Live human macrophages are shown to be able to rapidly uptake and accumulate ON in distinct cellular compartment specifically to ON size and charge. We show that the smallest QDs specifically target histories in cell nuclei and nucleoli by a multistep process involving endocytosis, active cytoplasmic transport, and entering the nucleus via nuclear pore complexes. Treatment of the cells with an anti-microtubule agent nocodazole precludes ON cytoplasmic transport whereas a nuclear import inhibitor thapsigargin blocks OD import into the nucleus. These results demonstrate that the nonfunctionalized QDs exploit the cell's active transport machineries for delivery to specific intranuclear destinations.
|Uncontrolled Keywords:||CDTE QUANTUM DOTS; LONG-TERM; MEMBRANE-PROTEINS; LIVE CELLS; IN-VIVO; MACROPHAGES; GENE; PHAGOCYTOSIS; PROBES|
|Subjects:||Material Science > Functional and hybrid materials|
Biomedical Science > Nanobiotechnology
|Deposited On:||09 Sep 2009 11:40|
|Last Modified:||09 Sep 2009 11:40|
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