Cambi, Alessandra and Lidke, Diane S. and Arndt-Jovin, Donna J. and Figdor, Carl G. and Jovin, Thomas M. (2007) Ligand-conjugated quantum dots monitor antigen uptake and processing by dendritic cells. NANO LETTERS, 7 (4). pp. 970-977.
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The dendritic cell (DC) specific pathogen-uptake receptor (DC-SIGN) internalizes antigens for degradation and presentation onto MHC molecules. At the cell membrane, DC-SIGN forms nanoclusters that facilitate virus capture. However, internalized viruses, such as HIV-1, escape degradation. Here, we exploit ligand-conjugated, virus-sized, highly photostable quantum dots (QDs) to monitor in living cells antigen binding, entry, and trafficking. The antigen-coated QDs specific uptake and persistence in live DCs open the possibility for tracking antigen-presenting cells in vivo.
|Subjects:||Biomedical Science > Nanobiotechnology|
Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
|Deposited On:||10 Sep 2009 10:33|
|Last Modified:||10 Sep 2009 10:33|
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