Cambi, Alessandra and Lidke, Diane S. and Arndt-Jovin, Donna J. and Figdor, Carl G. and Jovin, Thomas M. (2007) Ligand-conjugated quantum dots monitor antigen uptake and processing by dendritic cells. NANO LETTERS, 7 (4). pp. 970-977.
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Abstract
The dendritic cell (DC) specific pathogen-uptake receptor (DC-SIGN) internalizes antigens for degradation and presentation onto MHC molecules. At the cell membrane, DC-SIGN forms nanoclusters that facilitate virus capture. However, internalized viruses, such as HIV-1, escape degradation. Here, we exploit ligand-conjugated, virus-sized, highly photostable quantum dots (QDs) to monitor in living cells antigen binding, entry, and trafficking. The antigen-coated QDs specific uptake and persistence in live DCs open the possibility for tracking antigen-presenting cells in vivo.
| Item Type: | Article |
|---|---|
| Subjects: | Biomedical Science > Nanobiotechnology Biomedical Science > Nanotechnology for human health Biomedical Science > Nanomedicine |
| ID Code: | 6194 |
| Deposited By: | IoN |
| Deposited On: | 10 Sep 2009 10:33 |
| Last Modified: | 10 Sep 2009 10:33 |
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