Zhao, Q. and Sigalov, G. and Dimitrov, V. and Dorvel, B. and Mirsaidov, U. and Sligar, S. and Aksimentiev, A. and Timp, G. (2007) Detecting SNPs using a synthetic nanopore. NANO LETTERS, 7 (6). pp. 1680-1685.
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Official URL: http://pubs.acs.org/doi/abs/10.1021/nl070668c
We have discovered a voltage threshold for permeation through a synthetic nanopore of dsDNA bound to a restriction enzyme that depends on the sequence. Molecular dynamic simulations reveal that the threshold is associated with a nanonewton force required to rupture the DNA-protein complex. A single mutation in the recognition site for the restriction enzyme, i.e., a single nucleotide polymorphism (SNP), can easily be detected as a change in the threshold voltage. Consequently, by measuring the threshold voltage in a synthetic nanopore, it may be possible to discriminate between two variants of the same gene (alleles) that differ in one base.
|Uncontrolled Keywords:||NANOMETER-DIAMETER PORE; SILICON-OXIDE NANOPORE; MOLECULAR-DYNAMICS; DNA; BINDING; TRANSLOCATION; TRANSPORT; ENERGY; ECORI|
|Subjects:||Biomedical Science > Nanobiotechnology|
|Deposited On:||10 Sep 2009 12:18|
|Last Modified:||10 Sep 2009 12:18|
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