Megyesi, Monika and Biczok, Laszlo and Jablonkai, Istvan (2008) Highly sensitive fluorescence response to inclusion complex formation of berberine alkaloid with cucurbituril. JOURNAL OF PHYSICAL CHEMISTRY C, 112 (9). pp. 3410-3416.
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The effect of inclusion complex formation on the fluorescence properties of berberine, a clinically important natural alkaloid, was studied using cucurbituril as macrocyclic host compound. The formation of a very stable 1: 1 inclusion complex led to about 500-fold fluorescence intensity enhancement, which facilitated the detection of berberine even below nanomolar concentration. Addition of NaCl caused a significant change in the association constant and the fluorescence characteristics of the complex, whereas the variation of the anion had a small effect. 1-Alkyl-3-methylimidazolium type ionic liquids altered the fluorescent properties of the berberine-cucurbituril complex much more efficiently than did NaCl. Time-resolved fluorescence studies showed ternary complex formation. Because berberine fluorescence is insensitive to pH and increases substantially upon inclusion in cucurbituril, the reversible self-assembly of this host-guest pair may find analytical application in enzyme assays.
|Uncontrolled Keywords:||fluorescence - enzyme assays - berberine - natural alkaloid|
|Subjects:||Biomedical Science > Nanobiotechnology|
|Deposited On:||24 Aug 2009 15:02|
|Last Modified:||24 Aug 2009 15:02|
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