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Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors

Okita, Keisuke and Nakagawa, Masato and Hong, Hyenjong and Ichisaka, Tomoko and Yamanaka, Shinya (2008) Generation of Mouse Induced Pluripotent Stem Cells Without Viral Vectors. SCIENCE, 322 (5903). pp. 949-953. ISSN 0036-8075 (print), 1095-9203 (online)

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Induced pluripotent stem ( iPS) cells have been generated from mouse and human somatic cells by introducing Oct3/4 and Sox2 with either Klf4 and c-Myc or Nanog and Lin28 using retroviruses or lentiviruses. Patient- specific iPS cells could be useful in drug discovery and regenerative medicine. However, viral integration into the host genome increases the risk of tumorigenicity. Here, we report the generation of mouse iPS cells without viral vectors. Repeated transfection of two expression plasmids, one containing the complementary DNAs ( cDNAs) of Oct3/4, Sox2, and Klf4 and the other containing the c- Myc cDNA, into mouse embryonic fibroblasts resulted in iPS cells without evidence of plasmid integration, which produced teratomas when transplanted into mice and contributed to adult chimeras. The production of virus-free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine.

Item Type:Article
Subjects:Biomedical Science > Nanobiotechnology
ID Code:613
Deposited By:M T V
Deposited On:04 Dec 2008 14:41
Last Modified:04 Dec 2008 14:41

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