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Polarity orientation of microtubules utilizing a dynein-based gliding assay

Yokokawa, Ryuji and Murakami, Tetsutaro and Sugie, Takeshi and Kon, Takahide (2008) Polarity orientation of microtubules utilizing a dynein-based gliding assay. NANOTECHNOLOGY, 19 (12).

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Official URL: http://iopscience.iop.org/0957-4484/19/12/125505;j...

Abstract

The motor protein dynein was introduced into a nanotransport system. We oriented microtubules by their polarity, and immobilized them based on a dynein - microtubule gliding assay system. This system achieved unidirectional transport of kinesin-coated microbeads. In contrast to conventional kinesin-based orientation systems, the dynein-based system allowed the reverse motion of microtubules, resulting in an inversion of the orientation of microtubule polarity and thus reverse transport of kinesin-coated microbeads. This combined kinesin-and dynein-based system constitutes a new means to facilitate the bidirectional orientation of microtubules and transport of cargos in a nanofluidic system.

Item Type:Article
Subjects:Biomedical Science > Nanobiotechnology
Biomedical Science > Nanomedicine
ID Code:6119
Deposited By:IoN
Deposited On:12 Apr 2010 15:09
Last Modified:12 Apr 2010 15:09

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