Liao, Wei-Yin and Liu, Chung-Chiun and Wang, Christina (2008) Detection of lipoprotein-associated phospholipase A(2) using a nano-iridium particle catalyst-based biosensor. SENSORS AND ACTUATORS B-CHEMICAL, 134 (2). pp. 993-999.
Full text is not hosted in this archive but may be available via the Official URL, or by requesting a copy from the corresponding author.
Abstract
The detection and quantification of lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) using an iridium-modified carbon-based biosensor was successfully accomplished in this study. The detection procedure was based on the hydrolysis of short-chain carboxylic esters: which produced as products, organic acids and alcohol. The alcohol was then oxidized by alcohol oxidase producing hydrogen peroxide, which was detected electrochemically. A methyl ester (methyl butyrate) was chosen as the substrate for this Lp-PLA(2) study. The methanol produced from the methyl ester hydrolysis reaction was oxidized by alcohol oxidase, resulting in the production of hydrogen peroxide, which was quantified using the iridium-modified carbon-based biosensor. The detection of Lp-PLA(2), in the concentration range 0-150 U ml(-1), was established as following a linear relationship with a sensitivity of 1.45 nA U-1 in bovine serum. The effects of potential interfering species, such as uric acid (UA) and ascorbic acid (AA), are experimentally assessed. (c) 2008 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Lipoprotein-associated phospholipase A(2); Hydrolysis; Ester; Biosensor |
| Subjects: | Analytical Science > Nanotechnology for sensing and actuating |
| ID Code: | 610 |
| Deposited By: | M T V |
| Deposited On: | 04 Dec 2008 15:36 |
| Last Modified: | 04 Dec 2008 15:36 |
Repository Staff Only: item control page
