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Detection of lipoprotein-associated phospholipase A(2) using a nano-iridium particle catalyst-based biosensor

Liao, Wei-Yin and Liu, Chung-Chiun and Wang, Christina (2008) Detection of lipoprotein-associated phospholipase A(2) using a nano-iridium particle catalyst-based biosensor. SENSORS AND ACTUATORS B-CHEMICAL, 134 (2). pp. 993-999.

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Abstract

The detection and quantification of lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) using an iridium-modified carbon-based biosensor was successfully accomplished in this study. The detection procedure was based on the hydrolysis of short-chain carboxylic esters: which produced as products, organic acids and alcohol. The alcohol was then oxidized by alcohol oxidase producing hydrogen peroxide, which was detected electrochemically. A methyl ester (methyl butyrate) was chosen as the substrate for this Lp-PLA(2) study. The methanol produced from the methyl ester hydrolysis reaction was oxidized by alcohol oxidase, resulting in the production of hydrogen peroxide, which was quantified using the iridium-modified carbon-based biosensor. The detection of Lp-PLA(2), in the concentration range 0-150 U ml(-1), was established as following a linear relationship with a sensitivity of 1.45 nA U-1 in bovine serum. The effects of potential interfering species, such as uric acid (UA) and ascorbic acid (AA), are experimentally assessed. (c) 2008 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:Lipoprotein-associated phospholipase A(2); Hydrolysis; Ester; Biosensor
Subjects:Analytical Science > Nanotechnology for sensing and actuating
ID Code:610
Deposited By:M T V
Deposited On:04 Dec 2008 15:36
Last Modified:04 Dec 2008 15:36

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