Capdevila, Antonieta and Burk, Raymond F. and Freedman, Joshua and Frantzen, Frank and Alfbeim, Ingrid and Wagner, Conrad (2007) A simple rapid immunoassay for S-adenosylhomocysteine in plasma. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 18 (12). pp. 827-831. ISSN 0955-2863
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Official URL: http://www.jnutbio.com/article/S0955-2863(07)00046...
The measurement of plasma S-adetiosylhomocysteine is a more sensitive indicator of the risk for vascular disease than is plasma homocysteine. Because the level of S-adenosylhomocysteine is normally in the nanomolar range, it has been difficult to measure and necessitated the development of complex fluorometric and mass-spectrophotometric methods. We have now adapted an existing immunoassay used for the measurement of homocysteine to the measurement of S-adenosylhomocysteine in plasma. This assay is sensitive down to the level of less than 0.1 pmol, and there is no interference by S-adenosylmethionine. The assay is carried out in microplates, allows the measurement of 12 samples per plate and can easily be carried out in a 4-h period. The method is applicable to plasma samples having S-adenosylhomocysteine concentrations ranging from 10 to 150 nM without dilution. The mean value for 16 normal subjects by this method was 18.9 +/- 1.4 nM (S.E.M.), compared with 17.8 +/- 1.4 nM obtained by a previously described method using two high-performance liquid chromatography columns with fluorescence derivatization. Mean values for seven cirrhotic patients were 46.5 +/- 3.3 nM by this new method compared with 44.6 +/- 5.3 by the former method. The ease and speed of this method should allow the widespread measurement of this important metabolite in laboratories without access to sophisticated equipment. Published by Elsevier Inc.
|Uncontrolled Keywords:||S-adenosylhomocysteine; immunoassay; plasma; vascular disease; human|
|Subjects:||Biomedical Science > Nanomedicine|
|Deposited By:||M T V|
|Deposited On:||10 Dec 2008 09:06|
|Last Modified:||10 Dec 2008 09:06|
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