Nano Archive

DNA origami design of dolphin-shaped structures with flexible tails

Andersen, Ebbe S. and Dong, Mingdong and Nielsen, Morten M. and Jahn, Kasper and Lind-Thomsen, Allan and Mamdouh, Wael and Gothelf, Kurt V. and Besenbacher, Flemming and Kjems, Jorgen (2008) DNA origami design of dolphin-shaped structures with flexible tails. ACS NANO, 2 (6). pp. 1213-1218.

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The DNA origami method allows the folding of long, single-stranded DNA sequences into arbitrary two-dimensional structures by a set of designed oligonucleotides. The method has revealed an unexpected strength and efficiency for programmed self-assembly of molecular nanostructures and makes it possible to produce fully addressable nanostructures with wide-reaching application potential within the emerging area of nanoscience. Here we present a user-friendly software package for designing DNA origami structures ( and demonstrate its use by the design of a dolphin-like DNA origami structure that was imaged by high-resolution AFM in liquid. The software package provides automatic generation of DNA origami structures, manual editing, interactive overviews, atomic models, tracks the design history, and has a fully extendable toolbox. From the AFM images, it was demonstrated that different designs of the dolphin tail region provided various levels of flexibility in a predictable fashion. Finally, we show that the addition of specific attachment sites promotes dimerization between two independently self-assembled dolphin structures, and that these interactions stabilize the flexible tail.

Item Type:Article
Uncontrolled Keywords:DNA origami; self-assembly; dynamics; design software; AFM (atomic force microscopy)
Subjects:Material Science > Nanofabrication processes and tools
Material Science > Bio materials
ID Code:6048
Deposited By:IoN
Deposited On:15 Jul 2009 16:09
Last Modified:15 Jul 2009 16:09

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