Beier, Hope T. and Cowan, Christopher B. and Chou, I-Hsien and Pallikal, James and Henry, James E. and Benford, Melodie E. and Jackson, Joseph B. and Good, Theresa A. and Cote, Gerard L. (2007) Application of surface-enhanced Raman spectroscopy for detection of beta amyloid using nanoshells. PLASMONICS, 2 (2). pp. 55-64.
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Currently, no methods exist for the definitive diagnosis of AD premortem. beta-amyloid, the primary component of the senile plaques found in patients with this disease, is believed to play a role in its neurotoxicity. We are developing a nanoshell substrate, functionalized with sialic acid residues to mimic neuron cell surfaces, for the surface-enhanced Raman detection of beta-amyloid. It is our hope that this sensing mechanism will be able to detect the toxic form of beta-amyloid, with structural and concentration information, to aid in the diagnosis of AD and provide insight into the relationship between beta-amyloid and disease progression. We have been successfully able to functionalize the nanoshells with the sialic acid residues to allow for the specific binding of beta-amyloid to the substrate. We have also shown that a surface-enhanced Raman spectroscopy response using nanoshells is stable and concentration-dependent with detection into the picomolar range.
|Uncontrolled Keywords:||surface-enhanced Raman spectroscopy; SERS; Raman spectroscopy; nanoshells; beta-amyloid; Alzheimer's disease; Congo red; self-assembled monolayer|
|Subjects:||Analytical Science > Microscopy and probe methods|
Biomedical Science > Nanotechnology for human health
Biomedical Science > Nanomedicine
|Deposited On:||12 Apr 2010 16:50|
|Last Modified:||12 Apr 2010 16:50|
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