Nano Archive

Porous silicon biosensor for detection of viruses

Rossi, Andrea M. and Wang, Lili and Reipa, Vytas and Murphy, Thomas E. (2007) Porous silicon biosensor for detection of viruses. BIOSENSORS & BIOELECTRONICS, 23 (5). pp. 741-745.

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Abstract

There is a growing need for virus sensors with improved sensitivity and dynamic range, for applications including disease diagnosis, pharmaceutical research, agriculture and homeland security. We report here a new method for improving the sensitivity for detection of the bacteriophage virus MS2 using thin films of nanoporous silicon. Porous silicon is an easily fabricated material that has extremely high surface area to volume ratio, making it an ideal platform for surface based sensors. We have developed and evaluated two different methods for covalent bioconjugation of antibodies inside of porous silicon films, and we show that the pore penetration and binding efficiency depend on the wettability of the porous surface. The resulting films were used to selectively capture dye-labeled MS2 viruses from solution, and a viral concentration as low as 2 x 10(7)plaque-forming units per mL (pfu/mL) was detectable by measuring the fluorescence from the exposed porous silicon film. The system exhibits sensitivity and dynamic range similar to the Luminex liquid array-based assay while outperforming protein micro-array methods. (c) 2007 Elsevier B.V. All rights reserved.

Item Type:Article
Uncontrolled Keywords:porous silicon; biosensor; fluorescence; MS2; antibody; bioconjugation
Subjects:Biomedical Science > Nanobiotechnology
Material Science > Nanostructured materials
ID Code:5994
Deposited By:IoN
Deposited On:24 Aug 2009 14:03
Last Modified:24 Aug 2009 14:03

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