Jiang, Zhi-Liang and Huang, Yu-Juan and Liang, Ai-Hui (2008) An immunonanogold resonance scattering-quenching probe for rapid and sensitive assay of microalbumin. JOURNAL OF FLUORESCENCE, 18 (2). pp. 563-571. ISSN 1053-0509 (Print) 1573-4994 (Online)
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A novel and sensitive immunonanogold resonance scattering (RS) spectral probe was obtained for rapid detection of microalbumin (Malb), using 10 nm gold nanaoparticle to label goat anti-human Malb. It was based on that the gold-labeled anti-Malb took place nonspecific aggregation and exhibited a strong RS peak at 577 nm in pH 5.2 C6H8O7-Na2HPO4 buffer solution containing polyethylene glycol ( PEG), and the immunocomplex formed after specific reaction of gold-labeled anti-Malb with Malb, which led to a decrease in the intensity of RS peak at 577 nm considerably. The decreased RS intensity at 577 nm (Delta I-577nm) was linear to the concentration of Malb in the range of 4-128 ng/mL, with a detection limit of 3.2 ng/mL. The proposed method was applied to detect Malb in healthy human urine samples with satisfactory results.
|Uncontrolled Keywords:||microalbumin; nanogold-labeled; resonance scattering quenching|
|Subjects:||Biomedical Science > Nanobiotechnology|
|Deposited By:||M T V|
|Deposited On:||10 Dec 2008 14:50|
|Last Modified:||10 Dec 2008 14:50|
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