Nano Archive

Self-contained biomolecular motor-driven protein sorting and concentrating in an ultrasensitive microfluidic chip

Lin, Chih-Ting and Kao, Ming-Tse and Kurabayashi, Katsuo and Meyhofer, Edgar (2008) Self-contained biomolecular motor-driven protein sorting and concentrating in an ultrasensitive microfluidic chip. NANO LETTERS, 8 (4). pp. 1041-1046.

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Official URL: http://pubs.acs.org/doi/full/10.1021/nl072742x

Abstract

We developed a molecular sorter that operates without external power or control by integrating the microtubule-based, biological motor kinesin into a microfluidic channel network to sort, transport, and concentrate molecules. In our devices, functionalized microtubules that capture analyte molecules are steered along kinesin-coated microchannel tracks toward a collector structure, concentrated, and trapped. Using fluorescent analyte molecules and nanoliter sample volumes, we demonstrated 14 fM sensitivity, even in the presence of high concentrations of other proteins.

Item Type:Article
Uncontrolled Keywords:MICROTUBULE MOVEMENTS; KINESIN; TRANSPORT; NANOSTRUCTURES; MOTILITY; CHANNELS; ASSAY; ATP
Subjects:Biomedical Science > Nanobiotechnology
ID Code:5948
Deposited By:IoN
Deposited On:16 Sep 2009 10:24
Last Modified:16 Sep 2009 10:24

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