Bossi, Mariano and Foelling, Jonas and Belov, Vladimir N. and Boyarskiy, Vadim P. and Medda, Rebecca and Egner, Alexander and Eggeling, Christian and Schoenle, Andreas and Hell, Stefan W. (2008) Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species. NANO LETTERS, 8 (8). pp. 2463-2468.
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Official URL: http://pubs.acs.org/doi/full/10.1021/nl801471d
By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques.
|Uncontrolled Keywords:||OPTICAL RECONSTRUCTION MICROSCOPY; STIMULATED-EMISSION; DIFFRACTION-LIMIT; SINGLE MOLECULES; RESOLUTION; PROBES; LOCALIZATION; PHOTOCHROMISM; SPECTROSCOPY; RHODAMINE|
|Subjects:||Analytical Science > Microscopy and probe methods|
|Deposited On:||16 Sep 2009 10:30|
|Last Modified:||16 Sep 2009 10:30|
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