Hoshino, Akiyoshi and Nagao, Tomokazu and Nakasuga, Akira and Ishida-Okawara, Akiko and Suzuki, Kazuo and Yasuhara, Masato and Yamamoto, Kenji (2007) Nanocrystal quantum dot-conjugated anti-myeloperoxidase antibody as the detector of activated neutrophils. IEEE TRANSACTIONS ON NANOBIOSCIENCE, 6 (4). pp. 341-345.
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Fluorescent nanocrystal quantum dots (QDs) have been applied to a wide range of biological studies by taking advantage of their fluorescence properties. Here we show that QDs conjugated with antibody against neutrophil peroxidase, myeloperoxidase (MPO). We designed a novel method to conjugate QDs to antibody without losing any antibody function including their antigen recognizing and Fc-receptor binding activities. When we applied anti-MPO antibody (Ab) with conventional organic probes in the case of immunostaining of living cells, the antibodies lost their fluorescence because of MPO enzymic activity to produce reactive oxygen species. Our QD-conjugated anti-MPO (alpha-MPO-QDs) can detect MPO on the surface of activated neutrophils. In addition, anti-MPO-QDs did not react to the inactivated neutrophils. In conclusion, we demonstrated that antibody visualized the expression of MPO on the neutrophil surface after stimulation with proinflammatory cytokines. Taken together, these techniques have the possibility that QDs can reveal the activation of neutrophils by immunostaining and flow cytometric analysis as a powerful tool for diagnosis of the neutrophil activation in vitro.
|Uncontrolled Keywords:||quantum dot conjugated with antibody; bioimaging; diagnosis of activated neutrophils|
|Subjects:||Physical Science > Nano objects|
Biomedical Science > Nanobiotechnology
|Deposited On:||05 Aug 2009 10:15|
|Last Modified:||05 Aug 2009 10:15|
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