Nano Archive

Electrical detection of fast reaction kinetics in nanochannels with an induced flow

Schoch, Reto B. and Cheow, Lih Feng and Han, Jongyoon (2007) Electrical detection of fast reaction kinetics in nanochannels with an induced flow. NANO LETTERS, 7 (12). pp. 3895-3900.

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Official URL: http://pubs.acs.org/doi/full/10.1021/nl0724788

Abstract

Nanofluidic channels can be used to enhance surface binding reactions, since the target molecules are closely confined to the surfaces that are coated with specific binding partners. Moreover, diffusion-limited binding can be significantly enhanced if the molecules are steered into the nanochannels via either pressure-driven or electrokinetic flow. By monitoring the nanochannel impedance, which is sensitive to surface binding, low analyte concentrations have been detected electrically in nanofluidic channels within response times.of 1-2 h. This represents a similar to 54 fold reduction in the response time using convective flow compared to diffusion-limited binding. At high-flow velocities, the presented method of reaction kinetics enhancement is potentially limited by force-induced dissociations of the receptor-ligand bonds. Optimization of this scheme could be useful for label-free, electrical detection of blomolecule binding reactions within nanochannels on a chip

Item Type:Article
Uncontrolled Keywords:PLL-G-PEG; OXIDE SURFACES; POLY(L-LYSINE)-G-POLY(ETHYLENE GLYCOL); NANOFLUIDIC CHANNELS; PROTEIN ADSORPTION; ION-TRANSPORT; STREPTAVIDIN; ARCHITECTURE; RESISTANCE; CHARGE
Subjects:Physical Science > Nano objects
Biomedical Science > Nanobiotechnology
ID Code:5929
Deposited By:IoN
Deposited On:16 Sep 2009 10:41
Last Modified:16 Sep 2009 10:41

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