Nano Archive

Inducible RNA interference-mediated gene silencing using nanostructured gene delivery arrays

Mann, David G. J. and McKnight, Timothy E. and McPherson, Jackson T. and Hoyt, Peter R. and Melechko, Anatoli V. and Simpson, Michael L. and Sayler, Gary S. (2008) Inducible RNA interference-mediated gene silencing using nanostructured gene delivery arrays. ACS NANO, 2 (1). pp. 69-76.

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Official URL: http://pubs.acs.org/doi/abs/10.1021/nn700198y

Abstract

RNA interference (RNAi) has become a powerful biological tool over the past decade. In this study, a tetracycline-inducible small hairpin RNA (shRNA) vector system was designed for silencing cyan fluorescent protein (CFP) expression and delivered alongside the yfp marker gene into Chinese hamster ovary cells using impalefection on spatially indexed vertically aligned carbon nanofiber arrays (VACNFs). The VACNF architecture provided simultaneous delivery of multiple genes, subsequent adherence and proliferation of interfaced cells, and repeated monitoring of single cells over time. Following impalefection and tetracycline induction, 53.1% ± 10.4% of impalefected cells were fully silenced by the inducible CFP-silencing shRNA vector. Additionally, efficient CFP silencing was observed in single cells among a population of cells that remained CFP-expressing. This effective transient expression system enables rapid analysis of gene-silencing effects using RNAi in single cells and cell populations.

Item Type:Article
Uncontrolled Keywords:RNA interference; shRNA; nanostructures; nanofibers; gene silencing; vertically aligned carbon nanofibers; DNA delivery; tetracycline induction; single cell expression; CFP; YFP
Subjects:Physical Science > Nano objects
Biomedical Science > Nanobiotechnology
ID Code:5919
Deposited By:IoN
Deposited On:29 Jul 2009 12:22
Last Modified:29 Jul 2009 12:22

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