Nano Archive

Digital microfluidics for cell-based assays

Barbulovic-Nad, Irena and Yang, Hao and Park, Philip S. and Wheeler, Aaron R. (2008) Digital microfluidics for cell-based assays. LAB ON A CHIP, 8 (4). pp. 519-526.

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We introduce a new method for implementing cell-based assays. The method is based on digital microfluidics (DMF) which is used to actuate nanolitre droplets of reagents and cells on a planar array of electrodes. We demonstrate that this method is advantageous for cell-based assays because of automated manipulation of multiple reagents in addition to reduced reagent use and analysis time. No adverse effects of actuation by DMF were observed in assays for cell viability, proliferation, and biochemistry. A cytotoxicity assay using Jurkat T-cells was performed using the new method, which had similar to 20 times higher sensitivity than a conventional well plate assay. These results suggest that DMF has great potential as a simple yet versatile analytical tool for implementing cell-based assays on the microscale.

Item Type:Article
Uncontrolled Keywords:microfluidics - cell based assay - nanolitre - nanolitre droplets
Subjects:Biomedical Science > Nanobiotechnology
ID Code:5901
Deposited By:IoN
Deposited On:03 Sep 2009 16:05
Last Modified:03 Sep 2009 16:05

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