Lo, Chun-Liang and Lin, Ko-Min and Hsiue, Ging-Ho (2005) Preparation and characterization of intelligent core-shell nanoparticles based on poly(d,l-lactide)-g-poly(N-isopropyl acrylamide-co-methacrylic acid). Journal of Controlled Release, 104 (3). 477 - 488.
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Official URL: http://www.sciencedirect.com/science/article/B6T3D...
New thermo-responsive, pH-responsive, and biodegradable nanoparticles comprised of poly(d,l-lactide)-graft-poly(N-isopropyl acrylamide-co-methacrylic acid) (PLA-g-P(NIPAm-co-MAA)) were developed by grafting biodegradable poly(d,l-lactide) onto N-isopropyl acrylamide and methacrylic acid. A core-shell type nano-structure was formed with a hydrophilic outer shell and a hydrophobic inner core, which exhibited a phase transition temperature above 37 °C suitable for biomedical application. Upon heating above the phase transition temperature, PLA-g-P(NIPAm-co-MAA) nanoparticle showed a polarity increase of pyrene in either buffer solution or intra-hepato-carcinoma cells as determined by fluorescence measurement, indicating that the structure of nanoparticles caused leakages from outer shell copolymers aggregation and collapsed. The drug loading level of 5-fluorouracil (5-FU) encapsulated in the PLA-g-P(NIPAm-co-MAA) nanoparticles can be as high as 20%. The release of 5-FU from nanoparticles was strongly controlled by the pH in the aqueous solution. Based on these results, PLA-g-P(NIPAm-co-MAA) nanoparticles can be used as a drug carrier for intracellular delivery of anti-cancer drug.
|Uncontrolled Keywords:||Intelligent nanoparticle; Graft copolymer; Structural changes; Intracellular drug delivery; 5-Fluorouracil|
|Subjects:||Material Science > Nanofabrication processes and tools|
Material Science > Nanostructured materials
Biomedical Science > Nanomedicine
|Deposited On:||07 Jul 2009 16:44|
|Last Modified:||07 Jul 2009 16:44|
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