Maignien, Thomas and Shakweh, Monjed and Calvo, Pilar and Marcé, Dominique and Salès, Nicole and Fattal, Elias and Deslys, Jean-Philippe and Couvreur, Patrick and Lasmezas, Corinne Ida (2005) Role of gut macrophages in mice orally contaminated with scrapie or BSE. International Journal of Pharmaceutics, 298 (2). 293 - 304.
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Official URL: http://www.sciencedirect.com/science/article/B6T7W...
While there is a growing consensus on the understanding of the propagation pathways after oral infection of transmissible spongiform encephalopathy (TSE) agents and even if the central role of follicular dendritic cells is identified, little is known about the key players in the first steps of the infection and about the site of the disease development. We investigated the role of gut macrophages, which are capable of capturing aggregates of the prion protein. PLGA particles containing clodronate were designed in order to be orally administered and to target Peyer's patches for inducing gut-associated macrophages suicide in mice. Mice were subsequently infected with scrapie or BSE by the oral route. It was found that the efficacy of macrophage suppression in the Peyer's patches correlated well with an earlier appearance of PrPres in these formations and with a higher amount of PrPres at a later stage of the infection. Thus, the capture of infectious particles that have crossed the epithelial gut barrier and their elimination by macrophages seems to be a key event to restrict the amount of agent initiating the infection.
|Additional Information:||Selected contributions from the 5th European Workshop on Particulate Systems|
|Uncontrolled Keywords:||Transmissible spongiform encephalopaties; Macrophage-suicide technique; Clodronate; Peyer's patches; Intestinal uptake; Nano- and microparticles|
|Subjects:||Material Science > Nanostructured materials|
Biomedical Science > Nanomedicine
|Deposited On:||07 Jul 2009 17:00|
|Last Modified:||07 Jul 2009 17:00|
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