Nano Archive

A multifunctional envelope-type nano device for novel gene delivery of siRNA plasmids

Moriguchi, Rumiko and Kogure, Kentaro and Akita, Hidetaka and Futaki, Shiroh and Miyagishi, Makoto and Taira, Kazunari and Harashima, Hideyoshi (2005) A multifunctional envelope-type nano device for novel gene delivery of siRNA plasmids. International Journal of Pharmaceutics, 301 (1-2). 277 - 285.

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Official URL: http://www.sciencedirect.com/science/article/B6T7W...

Abstract

A multifunctional envelope-type nano device (MEND) for use in the delivery of siRNA expression plasmids is described. The plasmid DNA encoding anti-luciferase short interfering RNA (siRNA) was condensed by poly-l-lysine (PLL) and packaged into the MEND. The silencing effect of the MEND(PLL) showed a 96% inhibition of luciferase activities in a co-transfection study. The silencing effect was maintained at more than 60%, even under the 100-fold diluted conditions. In the luciferase transformed cells, however, the MEND(PLL) showed no significant silencing effect (10%), indicating heterogeneity in transfection by the MEND(PLL). To solve this problem, the DNA condensing agents were optimized by comparing PLL, stearyl octaarginine (STR-R8) and protamine (Prot). No difference in silencing effect (95–97%) was found among these MENDs in a co-transfection study. However, the MEND(Prot) showed a 70% silencing effect in the transformed cells. These results suggest that the MEND(Prot) has less heterogeneity in transfection, while the MEND(PLL) and the MEND(STR-R8) have large heterogeneities. These results demonstrate that MEND(Prot) is a promising gene delivery system for siRNA expression plasmids with less heterogeneity associated with the transfection.

Item Type:Article
Uncontrolled Keywords:Non-viral gene delivery system; Multifunctional envelope-type nano device; siRNA plasmid; Protamine
Subjects:Biomedical Science > Nanomedicine
ID Code:5811
Deposited By:SPI
Deposited On:07 Jul 2009 17:25
Last Modified:07 Jul 2009 17:25

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