Nano Archive

Self-assembled nanoparticles based on glycol chitosan bearing 5[beta]-cholanic acid for RGD peptide delivery

Park, Jae Hyung and Kwon, Seunglee and Nam, Ju-Ock and Park, Rang-Woon and Chung, Hesson and Seo, Sang Bong and Kim, In-San and Kwon, Ick Chan and Jeong, Seo Young (2004) Self-assembled nanoparticles based on glycol chitosan bearing 5[beta]-cholanic acid for RGD peptide delivery. Journal of Controlled Release, 95 (3). 579 - 588.

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Official URL: http://www.sciencedirect.com/science/article/B6T3D...

Abstract

The synthetic peptide bearing Arg–Gly–Asp (RGD) sequence is considered to specifically bind to αvβ3 integrin expressed on endothelial cells in the angiogenic blood vessels, which provides a potential to inhibit the tumor growth. As a carrier for the RGD peptide, hydrophobically modified glycol chitosan (HGC) capable of forming nano-sized self-aggregates was prepared by the chemical conjugation of 5β-cholanic acid to the main backbone of glycol chitosan. The RGD peptide labeled with fluoresein isothiocyanate (FITC-GRGDS) was loaded into self-aggregates in three different conditions: simple mixing, sonication, and solvent evaporation methods. Of different methods applied, solvent evaporation method showed the most promising results for peptide loading, as judged by the yield (>70%) and loading efficiency (>75%). It was found that the presence of FITC-labeled peptides makes the self-aggregates to be compact, possibly due to the role of both hydrophobic FITC and peptides containing carboxylic acids that allow hydrogen bonding and electrostatic interaction with the primary amino groups in the main backbone of glycol chitosan. FITC-labeled peptides were released from self-aggregates in a physiological solution (pH 7.4) for up to 1 day. From the cell adhesion and migration assays, it was demonstrated that FITC labeling of peptides does not significantly deteriorate biological activity of the parent peptide drug (GRGDS). Overall, the self-aggregates loaded with FITC-GRGDS might be useful for monitoring or destroying the angiogenic vessels surrounding the tumor tissue.

Item Type:Article
Uncontrolled Keywords:Peptide drug delivery; RGD peptide; Self-aggregates; Glycol chitosan
Subjects:Material Science > Nanofabrication processes and tools
Biomedical Science > Nanomedicine
ID Code:5766
Deposited By:SPI
Deposited On:24 Jul 2009 09:24
Last Modified:24 Jul 2009 09:24

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