Fanali, Salvatore and Camera, Emanuela and Chankvetadze, Bezhan and D'Orazio, Giovanni and Quaglia, Maria Giovanna (2004) Separation of tocopherols by nano-liquid chromatography. Journal of Pharmaceutical and Biomedical Analysis, 35 (2). 331 - 337.
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Official URL: http://www.sciencedirect.com/science/article/B6TGX...
Nanoliquid chromatography (nano-LC) was used for the separation of tocopherols (δ-, γ-, α-TOH), α-tocopherol acetate (α-TOH-Ac) and an antioxidant compound, namely butylated hydroxytoluene (BHT) used to prevent TOHs autoxidation. The separation was carried out in a fused silica capillary of 100 μm I.D. and 375 μm O.D. packed in our laboratory with RP18 silica stationary phase of either 5- or 3-μm diameter (23-cm long). The mobile phase was composed by mixtures of methanol (MeOH), acetonitrile (MeCN) and water. Typical analyses time for the separation of all the five components of the mixture were 6–9 min depending on the composition of the mobile phase. Efficiency and resolution were strongly influenced by the particle diameter and the highest Rs and N/m values were observed using 3-μm RP18 particles. Experiments performed with capillaries packed with 3-μm RP18 particles provided good limit of detection (LOD) and limit of quantification (LOQ) (for δ-, γ-TOH, α-TOH-Ac were 4 and 8 μg/ml, while for α-TOH were 6 and 10 μg/ml, respectively). The optimized method was applied to extracts of serum and pharmaceutical preparation containing α-TOH and α-TOH-Ac.
|Additional Information:||10th International Meeting on Recent Developments in Pharmaceutical Analysis (RDPA 2003)|
|Uncontrolled Keywords:||Nano-LC; Tocopherols; Vitamin E; Drugs; Serum|
|Subjects:||Biomedical Science > Nanomedicine|
|Deposited On:||24 Jul 2009 09:49|
|Last Modified:||24 Jul 2009 09:49|
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