Nano Archive

N-acetyl histidine-conjugated glycol chitosan self-assembled nanoparticles for intracytoplasmic delivery of drugs: Endocytosis, exocytosis and drug release

Park, Ji Sun and Han, Tae Hee and Lee, Kuen Yong and Han, Sung Soo and Hwang, Jung Jin and Moon, Dae Hyuk and Kim, Sang Yoon and Cho, Yong Woo (2006) N-acetyl histidine-conjugated glycol chitosan self-assembled nanoparticles for intracytoplasmic delivery of drugs: Endocytosis, exocytosis and drug release. Journal of Controlled Release, 115 (1). 37 - 45.

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Official URL: http://www.sciencedirect.com/science/article/B6T3D...

Abstract

Nano-sized vesicular systems (nanoparticles), ranging from 10 nm to 1000 nm in size, have potential applications as drug delivery systems. Successful clinical applications require the efficient intracellular delivery of drug-loaded nanoparticles. Here we describe N-acetyl histidine-conjugated glycol chitosan (NAcHis-GC) self-assembled nanoparticles as a promising system for intracytoplasmic delivery of drugs. Because N-acetyl histidine (NAcHis) is hydrophobic at neutral pH, the conjugates formed self-assembled nanoparticles with mean diameters of 150−250 nm. In slightly acidic environments, such as those in endosomes, the nanoparticles were disassembled due to breakdown of the hydrophilic/hydrophobic balance by the protonation of the imidazole group of NAcHis. Cellular internalization and drug release of the pH-sensitive self-assembled nanoparticles were investigated by flow cytometry and confocal microscopy. NAcHis-GC nanoparticles internalized by adsorptive endocytosis were exocytosed or localized in endosomes. The amount of exocytosed nanoparticles was dependent on the pre-incubation time prior to removal of free nanoparticles from the culture media. Flow cytometry and confocal microscopy showed that NAcHis-GC nanoparticles released drugs into the cytosol and cell cycle analysis demonstrated that paclitaxel-incorporated NAcHis-GC nanoparticles were effective in inducing arrest of cell growth.

Item Type:Article
Uncontrolled Keywords:Nanoparticle; Histidine; Chitosan; Endocytosis; Exocytosis; Endosome; Paclitaxel; Cell cycle arrest
Subjects:Biomedical Science > Nanomedicine
ID Code:5761
Deposited By:SPI
Deposited On:24 Jul 2009 09:56
Last Modified:24 Jul 2009 09:56

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