Rogatsky, Eduard and Stein, Daniel T. (2007) High Dead Volume Pumps: Delay Time Reduction by Short-Term Low-Ratio Split Flow in LC/MS Applications. Journal of the American Society for Mass Spectrometry, 18 (2). 245 - 247.
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Official URL: http://www.sciencedirect.com/science/article/B6TH2...
The aim of our work was to develop a low-cost, simple and reliable solution to reduce LC/MS analysis time by compensating for limitations inherent to high dead volume standard HPLC pumps. In our approach, we utilized a temporary (sub-1 min) low ratio flow split (1:10) at 5 mL/min pump flow before the column. During this short period, 90% of the entire pump flow is delivered to waste and used for fast pump/system equilibration. Although full-time flow splitting is widely used in capillary/nano applications (usually with high split ratios in the hundreds or thousands), to our knowledge, this is the first time that short-term low-ratio flow splitting has been used in conventional LC/MS applications.
|Subjects:||Analytical Science > Metrology and standards in nanotechnology|
|Deposited On:||24 Jul 2009 15:39|
|Last Modified:||24 Jul 2009 15:39|
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