Manabe, Yoshiyuki and Sugimoto, Takanori and Kawasaki, Tomoyuki and Ueda, Minoru (2007) Nanometer-scale direct observation of the receptor for the leaf-movement factor in plant cell by a novel TEM probe. Tetrahedron Letters, 48 (8). 1341 - 1344.
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Official URL: http://www.sciencedirect.com/science/article/B6THS...
Abstract
Electron microscopy is a useful method for observing localization of some proteins in a cell. In this Letter, we report a nanometer-scale direct observation of the receptor for a bioactive substance of small molecular weight by using TEM (transmission electron microscopy). We developed a novel TEM probe compound (1) that is a modified leaf-movement factor with benzophenone as a photoaffinity group and FITC as an antigen that is recognized by a nano-gold bound anti-FITC antibody. By using probe 1, we revealed that the receptor for the leaf-movement factor of Cassia mimosoides is localized in the plasma membrane of the plant motor cell.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Nyctinasty; Leaf-opening substance; TEM; Nano-gold; TEM probe |
| Subjects: | Biomedical Science > Nanomedicine |
| ID Code: | 5732 |
| Deposited By: | SPI |
| Deposited On: | 27 Jul 2009 09:45 |
| Last Modified: | 27 Jul 2009 09:45 |
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