Nano Archive

Toxicity of nanosized and bulk ZnO, CuO and TiO2 to bacteria Vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus

Heinlaan, Margit and Ivask, Angela and Blinova, Irina and Dubourguier, Henri-Charles and Kahru, Anne (2008) Toxicity of nanosized and bulk ZnO, CuO and TiO2 to bacteria Vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus. CHEMOSPHERE, 71 (7). pp. 1308-1316. ISSN 0045-6535

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Abstract

As the production of nanoparticles of ZnO, TiO2 and CuO is increasing, their (eco)toxicity to bacteria Vibrio fischeri and crustaceans Daphnia magna and Thamnocephalus platyurus was studied with a special emphasis on product formulations (nano or bulk oxides) and solubilization of particles. Our innovative approach based on the combination of traditional ecotoxicology methods and metal-specific recombinant biosensors allowed to clearly differentiate the toxic effects of metal oxides per se and solubilized metal ions. Suspensions of nano and bulk TiO2 were not toxic even at 20 g l(-1). All Zn formulations were very toxic: L(E)C-50 (Mg l(-1)) for bulk ZnO, nanoZnO and ZnSO4 center dot 7H(2)O: 1.8, 1.9, 1.1 (V. fischeri); 8.8, 3.2, 6.1 (D. magna) and 0.24, 0.18, 0.98 (T. platyurus), respectively. The toxicity was due to solubilized Zn ions as proved with recombinant Zn-sensor bacteria. Differently from Zn compounds, Cu compounds had different toxicities: L(E)C-50 (Mg l(-1)) for bulk CuO, nano CuO and CuSO4: 3811, 79, 1.6 (V.fischeri), 165, 3.2, 0,17 (D. magna) and 95, 2.1, 0.11 (T. platyurus), respectively. Cu-sensor bacteria showed that toxicity to V. fischeri and T. platyurus was largely explained by soluble Cu ions. However, for Daphnia magna, nano and bulk CuO proved less bioavailable than for bacterial Cu-sensor. This is the first evaluation of ZnO, CuO and TiO2 toxicity to V. fischeri and T. platyurus. For nano ZnO and nano CuO this is also a first study for D. magna. (c) 2007 Elsevier Ltd. All rights reserved.

Item Type:Article
Uncontrolled Keywords:recombinant sensor bacteria; nanoparticles; metal oxides; antibacterial action; bioluminescence; bioavailability
Subjects:Risk > Environment, health and safety aspects of nanotechnology
ID Code:571
Deposited By:M T V
Deposited On:15 Dec 2008 12:45
Last Modified:15 Dec 2008 12:45

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