Nano Archive

Electrochemical DNA nano-biosensor for the study of spermidine-DNA interaction

Mehdinia, Ali and Kazemi, S. Habib and Bathaie, S. Zahra and Alizadeh, Abdolhamid and Shamsipur, Mojtaba and Mousavi, Mir Fazlollah (2009) Electrochemical DNA nano-biosensor for the study of spermidine-DNA interaction. Journal of Pharmaceutical and Biomedical Analysis, 49 (3). 587 - 593.

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An electrochemical DNA nano-biosensor is prepared by immobilization of double stranded DNA (dsDNA) onto a mixed self-assembled monolayer (SAM) composed of azide- and hydroxyl-terminated thiols. The SAMs- and dsDNA-modified gold electrodes were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The interaction of spermidine with DNA was studied by differential pulse voltammetry at the DNA-modified electrode. The decrease in the guanine oxidation peak current was used to study the interaction. The binding constant (K), obtained by differential pulse voltammetry, was 1.85 × 105 M−1. A linear dependence of the guanine peak currents was observed in the range of 1.6–70.4 μM spermidine, with a detection limit of 0.72 μM and r = 0.994 by using differential pulse voltammetry.

Item Type:Article
Uncontrolled Keywords:Nano-biosensor; Self-assembled monolayer; DNA; Spermidine; Differential pulse voltammetry
Subjects:Analytical Science > Nanotechnology for sensing and actuating
ID Code:5684
Deposited By:SPI
Deposited On:28 Jul 2009 15:24
Last Modified:28 Jul 2009 15:24

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