Wu, Jianhong and Liu, Wei and Xue, Chenbing and Zhou, Shunchang and Lan, Fengli and Bi, Lei and Xu, Huibi and Yang, Xiangliang and Zeng, Fan-Dian (2009) Toxicity and penetration of TiO2 nanoparticles in hairless mice and porcine skin after subchronic dermal exposure. Toxicology Letters, In Press, Uncorrected Proof . - . (In Press)
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Official URL: http://www.sciencedirect.com/science/article/B6TCR...
The present study investigated the penetration and potential toxicity of titanium dioxide (TiO2) nanoparticles following its dermal exposure in vitro and in vivo. In vitro, after exposure to isolated porcine skin for 24 h, titanium dioxide nanoparticles of carious sizes cannot penetrate through stratum corneum. Interestingly, when studied in vivo, quite different results were obtained. After topically applied on pig ear for 30 days, TiO2 nanomaterials (10 and 60 nm) can penetrate through horny layer, and be located in deep layer of epidermis. Furthermore, after 60-day dermal exposure in hairless mice, nano-TiO2 particles can penetrate through the skin, enter different tissues and induce diverse pathological lesions in the major organs. Notably, P25 (21 nm) TiO2 nanomaterials shows a more wide tissue distribution, even appeared in the brain without inducing any pathological changes. Among all organs examined, the skin and liver display the most severe pathological changes that correspond to the increases in SOD and MDA levels. These results suggest that the pathological lesions are likely to be mediated through the oxidative stress induced by the deposited nanoparticles. Accordingly, the collagen content expressed as HYP content are also significantly reduced in mouse skin samples, indicating that topical applied nano-TiO2 in skin for a prolonged time can induce skin aging. Together, the present study indicates that nanosize TiO2 may pose a health risk after dermal exposure over a relative long time period.
|Uncontrolled Keywords:||Toxicity; Penetration; Titanium dioxide; Nanoparticles; Dermal exposure; BALB/c hairless mice|
|Subjects:||NanoSafety > Environment, health and safety aspects of nanotechnology|
|Deposited On:||04 Aug 2009 12:24|
|Last Modified:||04 Aug 2009 12:24|
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